Several pieces of evidence supported Sutton's theory.
- Reproduction involves the initial union of only two cells, egg and sperm. If Mendel's model was correct, then their two gametes must make equal hereditary contributions. Sperm, however, contain little cytoplasm suggesting that the hereditary material must reside within the nuclei of gametes.
- The diploid individuals have two copies of each pair of homologous chromosomes gametes have only one. This observation was consistent with Mendel's model in which diploid individuals have two copies of each heritable gene and gametes have one.
- Chromosomes ate segregate during meiosis, and each pair of homologous orients on the metaphase plate independently of every other pair.
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