The three hypothesis of Dna replication were evaluated by Mathew Meselson and Franklin Stahl of the California Institute of Technology in 1958.
The grew bacteria in a medium containing heavy isotope of nitrogen, N15 which became incorporated into the bases of the bacterial DNA.
After several generations, the DNA of these bacteria denser than that of bacteria grown in a medium containing the isotope of nitrogen, N14
Meselson and Stahl then, tranferred the bacteria from the N15 mediun to the N14 medium and collected the DNA at various intervals.
They dissolved the DNA in Cesium Chloride and then spunt it at a very high speed in an ultra-centrifuge.
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